human il 19  (R&D Systems)

Journal: The Journal of Clinical Investigation

Article Title: IL-20RB mediates tumoral response to osteoclastic niches and promotes bone metastasis of lung cancer

doi: 10.1172/JCI157917

Figure Lengend Snippet: ( A ) Quantification of EdU + A549 cells with IL20RB overexpression and/or treatment of CM from murine bone marrow–derived osteoclasts (OC) for 24 hours. Representative images are shown on the right. OC CM was mixed with A549 culture medium at a 1:3 ratio. ( B ) Quantification of EdU + HBM1 cells with IL20RB knockdown and/or treatment with OC CM for 24 hours. ( C – E ) Organoid formation of A549 ( C ), HBM1 ( D ), and LLC ( E ) after treatment with OC CM. OC CM was mixed with organoid culture medium at a 1:3 ratio. ( F and G ) Organoid formation of A549 ( F ) and HBM1 ( G ) after treatment with OC CM and/or the IL-19–neutralizing antibody (10 μg/mL). ( H ) Organoid formation of LLC cells after treatment with recombinant IL-19 protein. ( I and J ) IL-19 secretion of osteoclasts after treatment with control F12K medium or CM from A549 ( I ) or HBM1 ( J ) with or without CSF2 knockdown. Scale bars: 100 μm. P values were obtained by 2-tailed unpaired t test. Data are represented as mean ± SD.

Article Snippet: The human IL-19 recombinant protein was from Peprotech (catalog 200-19-10).

Techniques: Over Expression, Derivative Assay, Knockdown, Recombinant, Control

Journal: The Journal of Clinical Investigation

Article Title: IL-20RB mediates tumoral response to osteoclastic niches and promotes bone metastasis of lung cancer

doi: 10.1172/JCI157917

Figure Lengend Snippet: ( A and B ) Phosphorylation of JAK1 and STAT3 in A549 with or without IL20RB overexpression ( A ) and HBM1 with or without IL20RB knockdown ( B ) after treatment with OC CM for 24 hours. OC CM was mixed with A549 culture medium at a 1:3 ratio. ( C ) STAT3-responsive reporter activity in A549 with or without IL20RB overexpression after treatment with OC CM for 24 hours. ( D and E ) Phosphorylation of JAK1 and STAT3 ( D ) and STAT3-responsive reporter activity ( E ) in A549 with or without IL20RB overexpression after treatment with OC CM and/or IL-19–neutralizing antibody (10 μg/mL) for 24 hours. ( F and G ) Phosphorylation of JAK1 and STAT3 in A549 with or without IL20RB overexpression ( F ) and HBM1 with or without IL20RB knockdown ( G ) after treatment with IL-19 recombinant protein (25 ng/mL) for 15 minutes. ( H ) STAT3-responsive reporter activity in A549 with or without IL20RB overexpression after treatment with IL-19 recombinant protein (25 ng/mL) for 15 minutes. ( I ) Correlation between IL-20RB expression and STAT3 phosphorylation in the SHTCH lung cancer cohort ( n = 19 patients). Representative immunostaining images are shown on the right. Scale bar: 100 μm. P values were obtained by 2-tailed unpaired t test ( C , E , and H ) and Pearson’s correlation analysis ( I ). Data are represented as mean ± SD.

Article Snippet: The human IL-19 recombinant protein was from Peprotech (catalog 200-19-10).

Techniques: Phospho-proteomics, Over Expression, Knockdown, Activity Assay, Recombinant, Expressing, Immunostaining

Journal: Frontiers in Immunology

Article Title: Cervico-Vaginal Inflammatory Cytokine and Chemokine Responses to Two Different SIV Immunogens

doi: 10.3389/fimmu.2020.01935

Figure Lengend Snippet: Protein standards and antibodies.

Article Snippet: Recombinant Human IL-10 (aa 19-178) Protein , 1064-IL , Novus/R&D System.

Techniques: Recombinant